ABSTRACT

The goal of this research was to determine the antibacterial activity of four different medicinal plant extracts including Tridax procumbens, Nigella sativa, Acalypha indica, Vitex negundo as well as the phytochemicals present in the extract. In this study, ethanol and methanol solvents were used for the plant extraction. The antibacterial activity of crude extracts of four plants was investigated using agar well diffusion method against Staphylococcus aureus, Enterococci sp. Pseudomonas aeruginosa, Escherichia coli, Klebsiella sp., and Proteus sp. by agar well diffusion method. Standard antibiotic Ciprofloxacin is used as a positive control. The best plant extract was analyzed qualitatively for the presence of phytochemicals. Both the methanolic and ethanolic extract of Nigella sativa seeds showed higher zone of inhibition against gram-positive bacteria viz. Staphylococcus aureus, Enterococci when compared to gram-negative organisms. Among the four plant extracts, N. sativa seeds extract showed effective inhibition against the tested organisms. Phytochemical analysis revealed that tannin, saponin, alkaloids, flavonoids, phenol were present in the methanolic extract of N. sativa but terpenoids was absent. Nsativa seeds extract might be helpful in preparing the alternative drug against the bacteria causing wound infection which are cost-effective and non-toxic to human. Keywords: Plant extracts, antibacterial activity, methanol, ethanol, wound pathogens
Publication date: 01/04/2023

https://ijbpas.com/pdf/2023/April/MS_IJBPAS_2023_7053.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.4.7053