ABSTRACT

For estimating L-cysteine from bulk and pharmaceutical formulations, a simple, rapid, accurate, and cost- powerful first-order UV-by-product spectrophotometric technique has been developed. The maximum absorbance of L-cysteine in phosphate buffer (pH 8.0) is determined to be 232nm. The first-order derivative of the equal spectrum discovered maxima at 232 nm and minima at 204 nm. With a correlation coefficient of 0.9998, the L-cysteine follows linearity inside the concentration range of 100-500?g/ml. When the proposed method was applied to find out the concentration of L-cysteine in bulk, the percent amount estimated at 99.83% was found to be in good agreement with the label claim. Recovery experiments at three different levels, 80 percent, 100 percent, and 120 percent, were used to test the method's accuracy. The percent recovery was discovered to be between 99.87 to 100.03 percent. The method's accuracy and reproducibility are indicated by the low percent R.S.D. values. The method's precision was examined in terms of intra-day, inter-day, and repeatability. The method is precise if the percent R.S.D. value is comparatively less than 2. The robustness was investigated with the help of two analysts. For routine analysis of L-cysteine in bulk, the above method was a quick and cost-effective quality-control tool. Keywords: L-cysteine, first-order derivative, quantitative determination, phosphate buffer (pH 8.0)
Publication date: 01/04/2023

https://ijbpas.com/pdf/2023/April/MS_IJBPAS_2023_7029.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.4.7029