ABSTRACT

Background: Cardiospermum helicacabum L. known as the balloon plant or love in puff, belongs to the family Sapindaceae, is a long lived, scrambling vine. C.halicacabum leaves contain some pharmaceutically important compounds like saponin, stigmasterol. Methods: In the present investigation an attempt has been made to study rapid micro propagation from nodal explant of C.halicacabum and to check the anatomical features in the invitro and exvitro level. The nodal segments were cultured on MS medium supplemented with various plant growth regulators. The main reason for the micro propagation of this species is its high medicinal value in ayurveda, siddha, homeopathic and unani. Results: Highest direct regeneration percentage is shown in MS+BAP (0.3 mg/l) and induction of friable the callus from the cut end of nodal explants showed in the hormonal combination of MS+BAP+2,4-D at 0.3+0.2/L produced green, compact callus. The minimum period for callus induction from nodal plant was 11 days. Anatomical studies in the exvitro and invitro studies revealed that the presence of 5-8 vascular bundles in stem, and pith is present in the centre. In leaves, the non-glandular and glandular trichomes are present in both cases. Also the contamination frequency was checked and showed 85% in the combination of MS+2,4D (0.03mg/l). Conclusion: The propagation method in Cardiospermum helicacabum is highly useful in the conservation and mass clonal propagation. It will definitely help in the horticultural and ornamental improvement of this medicinal plant and can lead to the analysis of secondary metabolites and bioactivity of C.halicacabum. Keywords: Cardiospermum halicacabum, Tissue culture, Sapindaceae, organogenesis, Callus
Publication date: 01/04/2023

https://ijbpas.com/pdf/2023/April/MS_IJBPAS_2023_7004.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.4.7004