ABSTRACT

Objective Isolation of hydroxy citric acid from the Zanthoxylum rhetsa Linn seeds schezwan pepper and to evaluate this Obesity-related Diabetes mellitus Methods The isolated compound of hydroxy citric acid obtained from Zanthoxylum rhetsa Linn seed by using the standard method and confirmed by using chemical test and spectral studies, this isolated compound was used to further studies like evaluation of glucose uptake assay by using cell lines like 3T3-L1 cell lines Molecular docking studied were performed on Human Aldol Reductase (PDB 3RX2), Human Mineralocorticoid receptor (PDB 3VHU) receptors using Auto dock 1.5.6. Results The isolated Compound identified as Hydroxy citric acid (HCA) showed significant inhibition in obesity-related parameters like 3T3 L1 cell line study for with Insulin increases glucose uptake, metabolism and storage in adipose tissue and skeletal muscle. Glucose uptake assay study of HCA showed that an increase in glucose uptake. Molecular Docking studies results show that the hydroxy citric acid bind to the active site region of human aldose reductase (PDB 3RX2), Human Mineralocorticoid receptor (PDB 3VHU) enzymes with good binding energy compared to standard compound of metformin. Conclusion The isolated compounds from Zanthoxylum rhetsa Linn based on results analysis of molecular docking and cell line studies compound so it can be effectively used for the treatment of Diabeties related obesity which is predicted based on results scores. Keywords: Autodock, Hydroxy citric acid, 3T3-L1 cell lines, Zanthoxylum rhetsa Linn.
Publication date: 01/02/2023

https://ijbpas.com/pdf/2023/February/MS_IJBPAS_2023_6859.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.2.6859