ABSTRACT

The current study was focused on giving a simple, accurate and rapid validated analytical method for the estimation of Ranolazine using different analytical methods which is designed to utilize UV- spectroscopy (Conventional) and HPTLC (Contemporary) techniques. Utilizing automatic HPTLC (Aetron) with ‘Spraylin’ software and the equipment which consists of separate sample applicator (Hamilton microlitre-100 ?L syringe) and separate densitometer (spot detector at 270 nm and 365 nm) can be said as a major asset of this research work. The technique also consists of other software ‘JUST TLC’ for the calculation of band width and area of the sample spots developed. Uv- Spectroscopy (Labindia) with ‘Uv-Win 5’ software was also utilized for the same study on Ranolazine. Buffer has been used as a major diluent in both given methods in sample preparation process where the methods were proved falling under green chemistry, eco-friendly and economical methods. The Calibration plot shown best regression over the concentration range of 10-50 ?g/ml which provides the proof that the method is linear and sensitive in estimating lower quantity of the sample. The sample application was done on Silica Gel 60 F254 plates and developed using mobile phase Toluene, Ethyl Acetate and Methanol in the ratio of 6: 2: 2 v/v. The developed method was validated as per ICH Q2 (R1) guidelines. System suitability, precision, LOD, LOQ were performed, calculated and reported and were found to be within specified limits. The recovery studies were performed and amount recovered was found between 98.20-102.20%. Keywords: Ranolazine, HPTLC, UV, method development, Validation
Publication date: 01/02/2023

https://ijbpas.com/pdf/2023/February/MS_IJBPAS_2023_6824.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.2.6824