ABSTRACT

Silver nanoparticles (AgNPs) have already been reported as a viable remedy for microbial activities. There are a number of traditional procedures that use chemical processes and are linked to environmental harm. Green AgNP synthesis has been reported as safe, low-cost, and ecologically sustainable. Based on certain qualities such as size, distribution, and shape, nanoparticles demonstrate completely new or better properties. With decreasing nanoparticle size, the surface-to- volume ratio of nanoparticles increases. This approach reduces silver ions from AgNO3 into nano- sized particles by using extracts from several plants. In this study, a mixture of several leaf extracts such as Tulsi (Ocimum sanctum), Neem (Azadirachta indica), and Giloy (Tinospora cordifolia) was successfully used for the biosynthesis of stable silver nanoparticles. Numerous techniques were employed to characterize and investigate the antimicrobial activity of synthesized AgNPs. Fourier- transform infrared spectroscopy was used to confirm the presence of plant-origin capping agents surrounding AgNPs. UV-Visible spectrophotometer showed absorbance peak in the range of 436- 446 nm, SEM images showed that the silver nanoparticles are well dispersed and FTIR revealed that phytochemicals like flavonoids, terpenoids and reducing agents present in leaf extract were the main entities to stabilize the synthesized nanoparticles. The zones of inhibition showed that the AgNPs had significant antibacterial action against strains of Gram-positive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa and Proteus vulgaris) bacteria. Results revealed that AgNPs having potent antimicrobial activity could be prepared using different pure plant extracts and their mixtures. Keywords: Silver Nanoparticles, Green synthesis, Ocimum sanctum, Azadirachta indica, Tinospora cordifolia
Publication date: 01/01/2023

https://ijbpas.com/pdf/2023/January/MS_IJBPAS_2023_6785.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.1.6785