ABSTRACT

The current study was aimed to develop a simple, accurate and reproducible Reverse Phase High Performance Liquid Chromatography (RP-HPLC) analytical method for simultaneous estimation of Rutin, Scopoletin and Luteolin in Ayurvedic formulation. The analytical method was developed using Oyster Base Deactivated Silica C18 (250mm X 4.6mm, 5?) column in tertiary mode with mobile phase comprising of 0.3% Orthophosphoric acid in Water: Methanol: Acetonitrile (65:10:25), at flow rate of 1 ml/min and detection was carried out at 254nm using a UV detector. The retention time for rutin, scopoletin and luteolin were found to be 3.61min, 4.59min and 8.73min respectively. Linearity of all three phytomarkers was found to be in the range of 400-1200 ppm. The correlation coefficient for rutin, scopoletin and luteolin were 0.9988, 0.9994 and 0.9994 respectively. Limit of Detection and Limit of Quantification values of rutin, scopoletin and luteolin were found to be 24.25?g/ml and 74.50?g/ml, 17.38?g/ml and 52.69?g/ml and 5.44?g/ml and 16.48?g/ml respectively. Precision studies were carried out using intra-day and inter-day intervals which showed relative standard deviation values less than 2.00%. The accuracy study was determined by standard recovery method. The recovery values of the rutin (100.94% - 101.20%), scopoletin (100.13% - 100.62%) and luteolin (100.87% - 101.13%) indicate the method to be accurate. The developed method can be used as standardization tool for evaluation of formulation containing rutin, scopoletin and luteolin as marker compounds. Keywords: Rutin, Scopoletin, Luteolin, Ayurvedic formulation, RP-HPLC, Validation
Publication date: 01/01/2023

https://ijbpas.com/pdf/2023/January/MS_IJBPAS_2023_6736.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.1.6736