ABSTRACT

Silkworm, Bombyx mori has been used for wide variety of application. In the recent past, the various components of silk such as, silk film, functionalized bio-materials, silk thread, silk protein have found their utility in epidermal recovery, bone formation, in surgery and drug delivery systems. This newer findings in pharmaceutical research have added new dimension to the silk production. The genetics of yield expression, shown to be of polygenic nature but it has been given less importance. The current study is aimed to characterize the markers associated with 5 selected yield traits, with selective RFLP (Restriction fragment length polymorphism) derived sequence–tagged site (STS) primers on the genomic DNA of silkworm germplasm stocks of different origin and diverse yield potential. The molecular characterization directed to recognized two markers exhibits significant association with the yield traits. The markers could classify the stocks according to yield potential, respectful of their origin. The importance of the STS primers is postulated in the situation of applying multiple regression models for recognize markers associated with yield expression and stability for molecular breeding work in B. mori for yield improvement. Three restriction banding patterns were recognized among the screened silkworm genetic resources and their scoring revealed high, moderate and low fecundity and growth groups aa, ab banding types were found in Bivoltine high yielding groups and ac, bc found in high yielding Multivoltine groups. The prothoracic gene of B. mori naturally is a polymorphic, the respective gene it was well characterized and cloned with PTTH locus encoded PTTH in silkworm. The three alleles which was identified can be diagnosed using the gene amplification as a result of their gene length 3rd and 4th introns varies depending on the genotype. Express Sequence Tag site technique was employed as an important molecular tool to identify productive breeds among promising silkworm genetic resources. Identified productive races based on growth and yield specific genes can be used as high productive/robust breeds by the breeders for marker assisted selection breeding to evolve robust breed for field utilization and augmentation of silk productivity not only in the pharmaceutical field but others as well. Keywords: Silkworm; EST; RFLP- (STS); molecular markers
Publication date: 01/01/2023

https://ijbpas.com/pdf/2023/January/MS_IJBPAS_2023_6733.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.1.6733