ABSTRACT

An analytical method consists of a detailed, stepwise list of instructions to be followed in the qualitative, quantitative or structural analysis of a sample for one or more analytes and using a specified technique. A facile and rapid isocratic reverse phase high performance liquid chromatography assay method has been developed for simultaneous estimation of fluconazole and tinidazole in bulk and tablet. A waters HPLC system with Empower software was used. Inertsil ODS Column with phosphate buffer of pH 3.0 and methanol in the ratio of (70:30 v/v) as mobile phase at flow rate of 1 ml/min was employed for the analysis. The column was maintained at ambient temperature (27 0C). The eluent was monitored using PDA detector at 210nm. The run time was found to be 8 min. The developed method was validated as per ICH (International Conference on Harmonisation) guidelines. The developed method was found to be linear over a workable drug concentration, accurate, precise and robust. This fast and inexpensive method is suitable for research laboratories as well as for quality control analysis in pharmaceutical industries. Keywords: RP-HPLC, isocratic, validation, fluconazole, tinidazole, precision
Publication date: 01/12/2022

https://ijbpas.com/pdf/2022/December/MS_IJBPAS_2022_6685.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.12.6685