ABSTRACT

The objective of present study was to screen and isolate protease producing actinomycetes bacteria from soil samples collected from Muthupet lagoon forest, Tamil Nadu, India. Total six actinomycetes colonies showed clear zone around the colony indicating protease activity. Among these, ISA1 isolate produced highest protease activity and was identified as Streptomyces indiaensis (ISA1) by morphological, biochemical and molecular test. Moreover, various physiological characters were studied like pH and temperature. The protease showed maximum activity at pH 6 and the temperature for maximum protease activity was found to be 35°C. In optimization, various nutrient supplementations were used such as carbon sources and Nitrogen sources. Highest protease productivity was observed in Maltose. At the same highest protease productivity in peptone as a nitrogen source. These properties suggest that protease from Streptomyces indiaensis (ISA1) could find potential application in various industries. Keywords: Protease, Actinomycetes, Streptomyces indiaensis, Standard Fermented Medium and Orange Peels powder Fermented Medium
Publication date: 01/12/2022

https://ijbpas.com/pdf/2022/December/MS_IJBPAS_2022_6656.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.12.6656