ABSTRACT

There is not a singal analytical methods appeared in the literature for the determination of Acebutalol Hydrochloride and Hydrochlorothiazide in combine tablet dosage form. Attempts were made to develop RP-HPLC method of Acebutalol Hydrochloride and Hydrochlorothiazide in bulk drug and Sectrazide tablet formulation. RP-HPLC method was developed and validated as per ICH guidelines using Column C18 (4.6mm x 250mm) and Acetonitrile:Phosphate buffer (pH 2.5) in the ratio 85:15 as mobile phase. Retention time of Acebutalol Hydrochloride and Hydrochlorothiazide was found to be 3.07 and 4.43 mins respectively at the wavelength 230nm and flow rate 1.0ml/min. Acebutalol Hydrochloride (Acbtl) and Hydrochlorothiazide (Hctz) individually follows the Beer-Lamberts law over concentration range 5-25?g/ml and 2-10 ?g/ml regression of coefficient was found to be r 2=0.998 and r2=0.999 respectively. The percentage recovery was found in the range of 98% to 102% at three different levels. The method was found to simple yet accurate, precise and reproducible and successfully applied for the routine quality control analysis of Acebutalol Hydrochloride and Hydrochlorothiazide in bulk drug as well as in tablet formulation as per ICH guidelines. The result of the analysis were validated statistically and were found to be satisfactory. Keywords: Acebutolol Hydrochloride, Hydrochlorothiazide, Simultaneous equation, HPLC, UV Spectrophotometer
Publication date: 01/11/2022

https://ijbpas.com/pdf/2022/November/MS_IJBPAS_2022_6584.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.11.6584