ABSTRACT

This study was aimed to evaluate the potential cytotoxic activity of methanolic extracts of fungal and bacterial compounds against HeLa cervical cancer cell line and HepG2 liver cancer cell line. The cytotoxic activity of the extract was evaluated on HeLa and HepG2 cell lines using MTT assay method. Based on the morphological characters and chemical tests, the isolated microorganisms were found to be Cladosporium species (fungus) and Streptococcus species (bacteria). Thin layer chromatography (TLC) was performed to purify the compound present in the extract. Different concentrations (1, 10, 25, 50?g/mL) of the crude and TLC purified extracts were treated onto HeLa and HepG2 cancer cells which resulted in significantly inhibiting the proliferation of the cancer cells in a dose dependent manner. The cell number in the treated wells were exponentially lower when trypan blue assay was performed. These results suggest that both the isolated microorganisms contain secondary metabolites that possess anticancer property and hence responsible for the inhibition of the proliferating cancer cells. Key words: Cladosporium sp., anti-proliferative, cytotoxic, metabolites, Streptococcus sp.
Publication date: 01/10/2022

https://ijbpas.com/pdf/2022/October/MS_IJBPAS_2022_6519.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.10.6519