ABSTRACT

Streptococcus pyogenes, armed with multiple virulence factors, is a known notorious human pathogen responsible for various invasive and non-invasive diseases. The traditional usage of medicinal plants to treat human infections is an ever-increasing source of bioactive molecules. The present study demonstrates the antimicrobial activity against standard bacterial strains and the inhibitory effects against Streptococcus pyogenes biofilm of fifteen ethnomedicinal plant extracts. Plants like Holarrhena pubescens (256 µg/ml), Rhododendron arboreum (64 µg/ml), Hydrocotyle javanica (128 µg/ml), and Mimosa diplotricha (128µg/ml) showed potential antibacterial activity. These also exhibit a concentrationdependent reduction (83 – 92%) in the biofilm formation of S. pyogenes (SF370), significantly reducing micro-colonies and adherent cells, as revealed by crystal violet and microscopic assays. The AlamarBlue assay reveals that these crude plant extracts do not possess any bacteriostatic or bactericidal activity at their respective MBIC level against S. pyogenes. The GC-MS analysis revealed sesquiterpenes, unsaturated fatty aldehydes, and many other bioactive molecules in the plant extracts. This analysis has strengthened the ethnomedicinal information on these plants towards antimicrobial properties and contributes to anti-biofilm activity. Keywords: MBIC, Streptococcus pyogenes, Biofilm Inhibition, Resazurin, MIC, GC-MS
Publication date: 01/10/2022

https://ijbpas.com/pdf/2022/October/MS_IJBPAS_2022_6418.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.10.6418