ABSTRACT

The presented work offers a validated gradient method based on RP-HPLC enabling fast quantification of Amlodipine and Atenolol simultaneously in tablet formulations. The whole analysis is founded upon RP-HPLC separation using ODS-A C18 (250 mm x 4.6 mm, particle size-5?m) column maintained at 35°C. The mobile phase comprises a buffer, acetonitrile, and methanol. 0.9ml/min flow rate was set together with 20?l of injection volume and the entire analysis has been carried out at 228 nm. The chromatogram showed that there were well-separated Atenolol and Amlodipine peaks. Over the concentration range of 25 to 75?g/ml for Atenolol and 2.5 to 7?g/ml for Amlodipine a linear relationship of the drug concentration with peak areas was observed. With the recovery between 99.88% to 101.66% for Atenolol and 99.34% to 102.54% for Amlodipine, the current approach is proven to be accurate. The approach demonstrated high precision as for both the drugs percent Relative standard deviation was below 2%. Atenolol and Amlodipine in tablet formulation can therefore be analyzed using this method, which is more convenient as well as reliable than the other methods. Keywords: Gradient, RP-HPLC, Simultaneous, Quantification
Publication date: 01/09/2022

https://ijbpas.com/pdf/2022/September/MS_IJBPAS_2022_6368.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.9.6368