ABSTRACT

Diabetes mellitus is a fast-growing health concern that affects people all over the world. Despite the availability of synthetic medications, there is always a desire to find herbal formulations owing to their relationship with adverse effects. Traditional healers in India employ Quercus infectoria as a folk medicine to reduce inflammation outcomes. The goal of this work is to evaluate the antidiabetic potential of the galls and extract and purify an ?glucosidase inhibitory substance. Antidiabetic potential was evaluated by performing the inhibition of glucose diffusion and inhibition of ?-amylase activity. This was followed by in silico studies to anticipate the binding interaction of the isolated compound with ?glucosidase using molecular docking studies. To purify the active component, a methanolic extract of Quercus infectoria galls was screened for ?-glucosidase inhibitory activity and separated into n-butanol, ethyl acetate, and water fractions. HPLC was used to quantify the isolated chemical. AutoDock 4.2.6 was used to conduct the molecular docking investigation. Rosmarinic acid was shown to be the active ingredient in Q. infectoria galls that inhibited ?glucosidase with an IC50 of 1.75 µg/ml. In addition, an in silico docking research revealed the possibility of rosmarinic acid and ?glucosidase binding. The anti-diabetic activity of Q. infectoria galls and the isolated component, rosmarinic acid, was confirmed by the findings. Keywords: ?-glucosidase, hyperglycemia, molecular docking, Quercus infectoria, rosmarinic acid
Publication date: 01/09/2022

https://ijbpas.com/pdf/2022/September/MS_IJBPAS_2022_6353.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.9.6353