ABSTRACT

The purpose of this study was to analyze the gel (pain relieving) by using the experimental design techniques and by different instruments. The first part of the project was to select a compound and suitable method for the analysis of the compound. The non-steroidal anti inflammatory drug (gel form) was selected because it is playing an important role in today’s life. The compound selected was Ibuprofen gel. The trade name of the product was NUROFEN 5% Gel manufactured by Crookes Healthcare Ltd. HPLC reverse phased method2 was used for this analytical study. By using the ultra violet spectrometry, the ?max of ibuprofen was found to be at 222 nm and this wavelength was subsequently used in the method to analyse the ibuprofen. In second part of the study, the ibuprofen gel analyze by using FTIR, the Quantitative analyses of ibuprofen gel, this performed on the IRE crystal of FTIR by using the diffusion reflectance method. In this method, gel was analyzed by casting film of gel on the IRE crystal and also by using physical clamping device. In this method also different concentrations of the drug were taken, from that calibration curve were obtained. Also in FTIR, the area of gel was taken and it puts in the given equation. And then FTIR reveals the quantity by using diffusion reflectance FTIR system. Interference from degraded products was observed in the chromatograms. The main study was to determine both HPLC and FTIR together and comparing the result with the quantity achieved. Keywords: HPLC, FTIR, IRE crystal, Ultraviolet Spectroscopy, Reverse phase chromatography
Publication date: 01/09/2022

https://ijbpas.com/pdf/2022/September/MS_IJBPAS_2022_6348.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.9.6348