ABSTRACT

Background: The present paper reports are the simple, accurate, rapid & precise RP-HPLC method for the simultaneous estimation of Perindopril erbumine & Amlodipine besylate in the bulk & in the formulated drug substance. Methodology: The analytical method of Reverse Phase Liquid chromatographic has been performed on a Kromasil C8 (4.6mm x 250mm, 5µ particle size) column with the Buffer (6.8g Potassium dihydrogen orthophosphate) & Acetonitrile in the ratio 59:41 with adjusted pH 2.6 with orthophosphoric acid as a mobile phase & column oven temperature 40°C. The mobile phase flow rate was adjusted to 1.0 ml/min. & the injection volume should be 10 µl. 210 nmwas used as the wavelength for the detection of the sample. Result: The retention time of Perindopril erbumine and Amlodipine Besylate were found to be 4.483 min. and 6.767 min respectively. The concentration range of linearity was observed from 20 % to 160 % of the nominal concentration of Perindopril erbumine & Amlodipine Besylate correlation coefficient was 0.999 for both drugs. The percent recovery was found within the limits of the acceptance criteria with an average recovery of 99.4 % for perindopril erbumine & 99.6 % for Amlodipine besylate. Conclusion: The % RSD below 2.0 shows the high precision of the proposed method. The method can be adopted for the routine analysis of simultaneous estimation of Perindopril erbumine & Amlodipine Besylate in pharmaceutical solid dosage form Keywords: RP-HPLC; UV detector; Amlodipine Besylate; Perindopril Erbumine; Solid oral dosage form (tablets)
Publication date: 01/09/2022

https://ijbpas.com/pdf/2022/September/MS_IJBPAS_2022_6175.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2022/11.9.6175