ABSTRACT

Tranexamic acid is synthetic lysine used to reduce bleeding disorders. TXA inhibits plasmin(ogen) binding to fibrin and reduces fibrinolysis. TXA antifibrinolytic activity is typically measured by clot lysis; however, effects on plasmin generation (PG) are unclear due to a lack of tools to measure PG in plasma. In this study, we developed and validated a method for the determination of TA in human serum by liquid chromatography with mass spectrometer. Serum sample (100 µL) was dehydronation with perchloric acid, and after pH adjustment, chromatographic separation was performed on a C18 column and isocratically eluted using a mobile phase consisting of ammonium acetate buffer (pH 3..8) /acetonitrile (95:5, v/v) at a flow rate of 200 µL /min. The total run time was 5 minutes. Detection and quantitation were performed with the mass spectrometer using multiple reaction monitoring mode with the ion transition m/z 158.1 to m/z 95.1 for TA and m/z 144.0 to m/z 81.1 for the internal standard (cis-4-aminocyclohexanecarboxylic acid). The results were linear over the concentration range of 0.1-100 ?g/mL of TA, with limit of quantitation of 0.03 ?g/mL. The intra-day and inter-day assay coefficient of variations for serum were less than 1.8% and 2.1%, respectively, and the recovery of added standard TA was 92.5 to 99.3%. In conclusion; a simple and sensitive LC-MS/MS method has been developed for the determination of TA in human serum. The method showed excellent linearity, sensitivity, recovery and precision. This method is suitable for clinical pharmacokinetic studies. Keywords: Tranexamic acid, LC-MS/MS, human serum
Publication date: 01/08/2022

https://ijbpas.com/pdf/2022/August/MS_IJBPAS_2022_6318.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.8.6318