ABSTRACT

Searching for novel antibiotics is the need of the hour to combat multidrug resistant pathogens. Hence, an attempt was made to isolate and identify promising antimicrobial metabolite producing actinomycetes from mangrove sediments of Machilipatnam, Andhra Pradesh, India along with the study of their antimicrobial potential. The samples collected from mangrove habitats were pretreated with calcium carbonate, diluted and plated on yeast extract malt extract dextrose (YMD) agar medium to isolate actinomycetes. Over all 10 strains designated as VLCH1 to VLCH-10 were isolated and screened for their antimicrobial activity. Among them, one strain VLCH-1 which exhibited high antimicrobial potential was identified as Streptomyces cinnamonensis VLCH-1 based on polyphasic taxonomy including micro-morphological, cultural, biochemical, physiological and molecular methods (Genbank accession number: MZ951165). The growth pattern of the isolate was studied by culturing it in YMD broth. The culture broth extracted with ethyl acetate was tested for antimicrobial activity and found to be active against pathogenic Gram-positive, and Gram-negative bacteria as well as fungi. It was evident from the present study that mangrove habitats of Machilipatnam serve as a good source for potent actinomycetes possessing broad spectrum antimicrobial activity. Keywords: Mangrove actinomycetes, Phylogenetic study, Streptomyces cinnamonensis VLCH-1, Antimicrobial activity
Publication date: 01/07/2022

https://ijbpas.com/pdf/2022/July/MS_IJBPAS_2022_6226.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.7.6226