ABSTRACT

Background: PTEN is one of the foremost typically mutated human growth suppressor cistrons involved at intervals the expansion and survival of cells, likewise as within the suppression of tumor formation. Loss of PTEN activity, either at the super molecule or genomic level, has been involving many primary and pathologic process malignancies moreover as malignant neoplastic disease and pathological process malignancies as well as breast cancer. Methods: Data were taken from cBioportal platform for cancer genomics therein we chose study of Breast Invasive malignant neoplastic disease of TCGA Pan-cancer Atlas, from that study we identified 227 genes of RAS-signaling pathway and downloaded mutation data and template RNA Expression, RSEM (Batch normalized from Illumina HiSeq_RNASeqV2) incorporates 918 samples of primary carcinoma samples within which fifty-seven samples showing PTEN mutation. Then we make data matrix file in line with iDEP.91 portal guidelines. Results: Heatmap showed clusters of genes across the samples explains the regulation of genes. K-means clustering identified five clusters based on Elbow plot in KEGG Enrichment. Cluster E showed the most variety factors enrichment in carcinoma pathways regulation. Conclusion: Once the PTEN factor is mutated in carcinoma, it causes several oncogenes and non-oncogenes to be triggered and suppressed. RASsignaling pathway activated tumor microenvironments which were interlinked with many pathways for supporting breast cancer tumor progression, cell growth and proliferation. Keywords: PTEN gene, Cancer, Oncogene, Breast cancer, India
Publication date: 01/06/2022

https://ijbpas.com/pdf/2022/June/MS_IJBPAS_2022_6854.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.6.6854