ABSTRACT

An accurate, specific ultra performance liquid chromatography-tandem mass spectroscopy (UPLC-MS/MS) method for the quantitative determination of curcumin using the human plasma. Curcumin D6 is used as an internal standard (IS). The compound was extracted from the plasma using Liquid-Liquid extraction technique. The organic solvent was evaporated and reconstituted in the mobile phase. The experiment was done using ACQUITY UPLC BEH 1.8 µm (2.1mm*100mm) column and time of flight mass spectrometer was used for the detection of compound equipped with electro spray ionization in Negative mode. The Mass lynx 1.4 software is used to obtain the data of the analysis. The mobile phase used here in combination of Acetonitrile and Buffer in the ratio of (90:10, v/v) with 0.1% formic acid and isocratic elution was used.The total run time was 2.50minthe analysis done for quantification using the MRM (Multiple Reaction Monitoring) with mass / charge (m/z) ratio parent ion of curcumin was 367.16 and curcumin D6 was 373.20, daughter ion of curcumin 148.99 and curcumin D6 was 176.180, the dwell time of curcumin 0.100 and curcumin D6 was 0.100, cone voltage of curcumin was 30 and curcumin D6 was 30. The retention time of curcumin and IS 1.45 and 1.4 mins respectively. The Bio analytical methods are accurate and reliable to determine these drugs at lower concentrations. Selectively Liquid Chromatography-Mass Spectrometry method to quantitate Curcumin in K?EDTA.Human plasma over the concentration range 0.4180 to 72.2360 ng/mL was successfully validated. This method is suitable for sample analysis to support bio-equivalence/bioavailability studies involving formulations of Curcumin. By ultra-performance Liquid chromatography UPLC– MS/MS is currently considered as the best choice. Curcumin is quantifiedthrough UPLCMS/MS and it has high specificity, more sensitivity, and increased rapidity. Keywords: Curcumin, UPLC–MS/MS, K?EDTA, Quantification, Bio-equivalence, Bioavailability
Publication date: 01/06/2022

https://ijbpas.com/pdf/2022/June/MS_IJBPAS_2022_6167.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.6.6167