ABSTRACT

Bio-analytical is the pathway for studying bioavailability and bioequvalence. The evaluation carried out by using some biological matrix loot. Here proper method development and validation is done with respective to guidelines. A sensitive and selective LC-MS/MS method to quantify Fluvoxamine in K2EDTA human plasma over the concentration range 2.6260 to 294.0960 ng/mL was developed and validated. Fluvoxamine and its internal standard (Fluvoxamine D4) were selectively extracted from 250 µL plasma by liquid-liquid extraction technique. Separation was achieved by liquid-liquid extraction on Hypurity C18 (50 mm x 4.6 mm, 5µm) column. The plasma samples (250 µL) were processed using liquid-liquid extraction technique. The developed work is specific LC-MS/MS procedure for the determination of Fluvoxamine in human plasma to support bioequivalence/bioavailability studies. Keywords: Bio-Analytical Method, UPLC-MS/MS, Coefficient Variation, Standard Deviation, Lower Limit of Quantification, Upper Limit of Quantification
Publication date: 01/05/2022

https://ijbpas.com/pdf/2022/May/MS_IJBPAS_2022_6101.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2022/11.5.6101