ABSTRACT

A stable, rapid, accurate and selective method has been developed for the estimation of Atorvastatin calcium and Celecoxib by using buffer (pH3.7) and acetonitrile in ratio(40:60 v/v) in combination as mobile phase and at the flow rate of 1.5 ml/min at ????max 254 nm. Chromatographic separation was performed on Shimadzu LC-2010 ATVP prominence liquid chromatograph and using Shimadzu SPD-10AVP UV-Visible detector, an Qualisil GOLD C18 5µ (250× 4.60 mm) column used as stationary phase. Validation of the method was done according to the guidelines ICH Q2 (R1). Calibration curve was linear at concentration of 1-10 µg/ml of Atorvastatin calcium and Celecoxib respectively; detection was carried out at ????max 254 nm; linear regression equation for Atorvastatin calcium was Y=33546x-192.6; R2=0.998 and linear regression equation for Celecoxib was Y=51830x-5320; R2=0.998 respectively. Retention time for Atorvastatin calcium and Celecoxib was 4.544 min and 6.064 min respectively. LOD for Atorvastatin calcium and Celecoxib was 0.01399 µg/ml and 0.01460 µg/ml respectively and LOQ for Atorvastatin calcium and Celecoxib was 0.0424µg/ml and 0.04426µg/ml respectively. The results of present study suggested that proposed method provides good peak resolution of Atorvastatin calcium and Celecoxib within short analysis time (<10 min) and high percentage of recovery shown that method is free from interference of excipients. The % RSD of each parameter lies below the limit of 2% proves the suitability. The statistical analysis proved that the proposed method is precise, accurate, selective and rapid for the estimation of both drugs. Keywords: Atorvastatin calcium, Celecoxib, RP-HPLC
Publication date: 01/05/2022

https://ijbpas.com/pdf/2022/May/MS_IJBPAS_2022_6096.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.5.6096