ABSTRACT

Background: The apricot kernel seeds contain amygdalin, which is considered as a naturally occurring anticancer agent. But recently it is debated that this compound does not positively have any anti-cancer effect. Aim: The aim of the study is to perform an in vitro study to assess the efficacy of apricot kernel seeds as an anticancer agent. Materials and methods: This study was conducted using KB cells procured from NCCS Pune and maintained in Duelbecco’s Modified Eagle’s medium. The cells were subcultures and were treated to apricot kernel seed extract at different concentrations 10, 50 and 100 ?g/ mL; in different duration 24 h, 48 h and 72 hours and the cells were evaluated for the viability, anti-proliferative and cell cycle progression. Result: The cells treated at higher concentrations 100 ?g/ mL of apricot kernel seed extract for longer duration revealed a hindrance in the proliferative effect of the cell predominantly in the S-phase of the cell cycle with few cells losing its viability. At low concentrations of the extract at shorter duration, there was a noticeable stimulatory effect for proliferation of the cells. Conclusion: There is positive anti-proliferative effect in the cells treated with apricot kernel seed extract but the viability of the cells is still evident with only few cells becoming non-vital. So, this proves that apricot kernel seeds have anti-cancer effect and thus, the protocol for anti-cancer therapies can be standardized using apricot kernel seeds. Keywords: Apricot kernel seed; Anti-cancer agent; In vitro study
Publication date: 01/05/2022

https://ijbpas.com/pdf/2022/May/MS_IJBPAS_2022_5874.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.5.5874