ABSTRACT

Attempts were made to accurate, precise and reproducible develop RP-HPLC method for estimation of Ondansetron and Ranitidine from tablet. For the RP HPLC Agilent (S.K) method Gradient System UV Detector and C18 column with 250mm x4.6 mm i.d and 5?m particle size methanol: 0.1% water with OPA (40:60v/v) was used as the mobile phase for the method. The detection wavelength was 245 nm and flow rate was 0.8 ml/min. In the developed method, the retention time of Ondansetron and Ranitidine were found to be 4.02 and 5.52 min. The developed method was validated according to the ICH guidelines. The linearity, precision, range, robustness was within the limits as specified by the ICH guidelines. Hence the method was found to be simple, accurate, precise, economic and reproducible. So, it is worthwhile that, the proposed methods can be successfully utilized for the routine quality control analysis Ondansetron and Ranitidine bulk drug as well as in formulations. Keywords: Ondansetron and Ranitidine, method development, validation, simultaneous estimation, RP- HPLC

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https://doi.org/10.31032/IJBPAS/2022/11.4.6033