ABSTRACT

To investigate the biodegradation of di-2-ethyl phthalate (DEHP) by an isolated bacterial strain Bacillus cereus spp. from soil contaminated with phthalate, collected from a garbage dumping site in Patna. The strain named T20 was isolated by serial dilution method in minimal salt media (MSM) supplemented with DEHP. We studied the effects of various environmental and chemical factors for optimising the conditions for degradation. The strain T20 was identified based on its phenotypic as well as phylogenetic characteristics by performing 16S rRNA gene sequencing and for the determination of the metabolic end products after degradation, liquid chromatography and mass spectrometry (LC-MS) analysis was done. The bacterial isolate was identified as Bacillus cereus (accession no.CP026375.1) by 16sr RNA gene sequencing analysis. According to Tukey post-hoc test the optimal temperature for degradation was 37 °C and pH was 7.0 and it could tolerate up to 0-5% NaCl. The most preferable carbon source was lactose and nitrogen source was yeast extract to optimize the conditions for DEHP degradation. Liquid chromatography and mass spectrometry (LC-MS) was done to estimate degradation intermediates of DEHP. Strain T20 showed potential characteristics making it a promising strain that can be implemented in the bioremediation of soil contaminated with phthalate. Keywords: Bioremediation, DEHP, LC-MS, phthalate degradation, 16s r-RNA
Publication date: 01/04/2022

https://ijbpas.com/pdf/2022/April/MS_IJBPAS_2022_6024.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.4.6024