ABSTRACT

Since the Bronze Age the synthetic dyes are prominent in the Indian textile industry. The uses of such synthetic dyes have many adverse effects in the environmental bodies. This study focuses on the ability of selected fungal isolates to decolorize and degrade ME4BL a widely used synthetic textile dye. The textile effluent was collected from the industrial zones of Surat. The reactive dye ME4BL was obtained from GIDC Ahmedabad. Various Fungi were isolated from textile effluent and screened by their capability to decolonize and decolorize the dye. Optimization of parameters for ME4BL dye decolourization was studied under static as well as shaking conditions. The static condition was proved better than the shaking condition in the degradation activity. The optimum pH and temperature for A4 fungi were 4 and 37ºC respectively. The UV - visible absorbance spectra and the HPLC analysis of the decolorized ME4BL significantly differed from those of the parent dye, indicating that the ME4BL was degraded by the fungal isolate. The conjugation of Silver nanoparticle and fungal isolates were highly effective in removal of the dye from effluents within a short period of time. The 18 ITS gene sequencing method was carried out in which strain A4 was identified as Aspergillus terrace. The degradation rate was reported as 74.35 % in 72 hrs. in 100 mg/L dye concentration. Key words: ME4BL dye, effluent, degradation, nanoparticles
Publication date: 01/04/2022

https://ijbpas.com/pdf/2022/April/MS_IJBPAS_2022_6019.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.4.6019