ABSTRACT

The present investigation is mainly focused on developing the genetic relationship between Alocasia indica and Tephrosia perpurea plant samples (05 each) collected from various zone of Chhattisgarh using fifteen 10 decamer random amplified polymorphic DNA (RAPD) molecular marker. The result revealed that A. indica had a similarity coefficient ranged from 0.23 to 0.43 whereas it ranged from 0.51 to 0.69 in case of T. perpurea plant. The dendrogram showed that all selected plants of A.indica grouped in one; except samples collected from Lundra Surguja which separated alone at a coefficient value of 0.23, it was seen that, sample collected from Achanakmar forest Bilaspur and Pakhanjur Kanker were firmly related together. Similarly 05 plants of T. perpurea grouped in a single cluster; except sample collected from Lundra Surguja which separated alone at a coefficient value of 0.51, sample collected from the University campus, Pt. RSU, Raipur and Pakhanjur Kanker was firmly related together. A set of differentiating markers viz. OPB-06, OPC-01, OPD-01 and OPD-03 may be used as diagnostic markers to identify the particular plant. The finding, suggested that genetic characterization be useful for distinguishing variation at molecular level and rapid identification of any plant species. Keywords: Alocasia indica, Tephrosia Purpurea, Molecular marker, RAPD, Genetic relationship
Publication date: 01/04/2022

https://ijbpas.com/pdf/2022/April/MS_IJBPAS_2022_5995.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.4.5995