ABSTRACT

Objective: The purpose of this study was to develop an RP-HPLC method for determining drug content and entrapment efficiency in ursolic acid-loaded solid lipid nanoparticles (UA-SLNs). Methods: The RP-HPLC method was carried out in the reverse phase (RP) C18 column with a mobile phase of methanol: water (80:20 v/v), at the flow rate of 1mL/min. The ?max was set at 249nm. Results: The retention time of ursolic acid was found at 5.7 ± 0.02 minutes. The approach was demonstrated to be specific, linear in the range of 0.5-8.0 µg/mL (R2 0.999), precise at the intraday and inter-day levels as evidenced by the relative standard deviation values (<1.0%), and accurate at the intra-day and inter-day levels (average recovery 99.02 to 99.59%). The detection and quantification limits were determined to be 0.074 and 0.228 µg/mL, respectively. The method was utilized to determine ursolic acid drug content and entrapment efficiency (EE) in solid lipid nanoparticles were found to be 98.82 ± 0.35% and 87.29 ± 1.12%. Conclusion: The RP-HPLC method may be useful for the determination of ursolic acid content and entrapment efficiency in solid lipid nanoparticles. Keywords: Ursolic acid, RP-HPLC, solid lipid nanoparticles, method development, and validation
Publication date: 01/03/2022

https://ijbpas.com/pdf/2022/March/MS_IJBPAS_2022_6411.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.3.6411