ABSTRACT

Background: In medical labs, storage of whole blood and other blood products such as serum or plasma is often necessary due to technical challenges or to reserve samples for future reasons such as research. The goal of this research was to look into and establish the stability of specific biochemical analytes, particularly creatinine, urea, and uric acid, under various storage time and temperature settings. Materials and procedures: Following storage, a total of three biochemical analytes in the serum of ten people were analyzed. Following the baseline measurements, each individual's serum was aliquoted and kept at 4°C for 4, 8, and 12 hours, as well as at -20°C for 7, 15, and 30 days, before being evaluated for stability. The findings were compared to the results of the original study, which were taken from fresh samples. Results: Serum uric acid levels become statistically insignificantly differing underneath all situations at the same time as serum urea and creatinine had been demonstrating instability following extended storage; tiers considerably decreased at 4 oC in eight,12 hrs and -20°C in 7, 15, and 30 days (P < 0.05). In conclusion: Urea and creatinine levels in serum were affected by different storage conditions while uric acid serum levels remained stable. Keywords: Urea, creatinine, uric acid, storage, temperature, baseline measurement
Publication date: 01/12/2021

https://ijbpas.com/pdf/2021/December/MS_IJBPAS_2021_DEC_SPCL_2051.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.12.2051