PHYTOCHEMICAL SCREENING AND STANDARDIZATION OF PUSHPA VARTI (A POLYHERBAL AYURVEDIC OPHTHALMIC FORMULATION) THROUGH HPTLC Authors: Shivkant Sharma*, Rajeev G. Dole
ABSTRACT
Many of the ophthalmic herbal formulations are described in classical literatures of Ayurvedathe traditional Indian system of medicine, as local applications in the form of vartis (Pills),
anjana (Collyrium), and eye drops etc. These formulations are very effective in the management
of eye diseases. Pushpavarti is a herbal ophthalmic formulation. It is used in the various eye
aliments like refractive errors, presbiopia, pterygium, cysts, scar, subconjunctival hemorrhage
and any growth in conjunctiva’s in the form of anjana (Collyrium). Pushpavarti constitutes four
drugs (dried flower of Sesamum indicum, and Jasminum grandiflorum and dried fruit of piper
longum and piper nigrum). The present study was aimed to screen the organoleptic analysis,
physico-chemical analysis, microbial test limit and phytochemical constituents through
preliminary phytochemical tests of Pushpa Varti and to standardize this poly herbal mixture
through High Performance Thin Layer Chromatography fingerprinting. The preliminary
phytochemical screening of the extract revealed the presence of bioactive compounds like
alkaloid, Tannins and polyphenols, saponins, flavonoids, steroids and Terpenoids. The HPTLC fingerprint profile, obtained from this study, of the same herbal formulation may be used for
authenticity and quality analysis.
Keywords: Ayurveda, Bioactive compounds, Pushpa Varti, Polyherbal formulation, Phytochemical
analyses, HPTLC fingerprinting, standardization
Publication date: 25/01/2022 https://ijbpas.com/pdf/2022/January/MS_IJBPAS_2022_JAN_SPCL_2_2054.pdfDownload PDFhttps://doi.org/10.31032/IJBPAS/2022/11.1.2054