ABSTRACT

Pioglitazone and Glimepride are used for the treatment of type-II diabetes mellitus. Accurate, rapid, simple, precise high performance thin layer chromatographic method (HPTLC) for Pioglitazone and Glimepride has been established and validated according to ICH guideline. Separation was achieved on silica gel 60 F254 plates with Toluene: Ethyl acetate: Diethyl ether (6:3:1v/v/v) used as mobile phase. The Rf values for Pioglitazone and Glimepride were found to be 0.88 and 0.79.Results were linear in range of 6000-12000 ng/mL for Pioglitazone and 800- 1600 ng/mL for Glimepride. The repeatability testing for sample and standard solutions were found as %RSD NMT 2.0% which was within the acceptable limits. Percentage recovery for Pioglitazone is 100.06% and for Glimepride is 99.94%. LOD for Pioglitazone and Glimepride was found to be 2.75ng/mL and 1.10ng/mL and LOQ for Pioglitazone and Glimepride was 8.34ng/mL and 3.33 ng/mL. The developed method was validated for linearity, precision, accuracy, robustness, limit of detection, limit of quantification according to ICH guideline. Keywords: HPTLC, Pioglitazone, Glimepride, LOD, LOQ, PIO, GLI
Publication date: 01/02/2022

https://ijbpas.com/pdf/2022/February/MS_IJBPAS_2022_5908.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2022/11.2.5908