ABSTRACT

Objective: In the present study the aqueous and ethanol extract of Ocimum basilicum seed was studied for anti-oxidant activities. Methods: The antioxidant activities were investigated using 1,1-diphenyl-2-picryl hydrazyl (DPPH) method, ABTS scavenging assay, hydrogen peroxide, superoxide anion radical scavenging assay and metal chelating ability on ferrous ions under in vitro condition. Results: The ethanolic extract of seed of Ocimum basilicum showed significant scavenging effect on DPPH (83.80±1.22) ABTS (74.85±0.824), Hydrogen peroxide (68.75 ±0.824), superoxide anion (78.37±0.808) and metal chelating (71.34 ±0.008) and the aqueous extract exhibited the inhibition for ABTS (63.78±0.824), hydrogen peroxide (54.69 ±0.824), superoxide anion (65.74±0.808) and metal chelating (65.74 ±0.008), DPPH (81.26 ±0.808) compared with standard drug. Conclusion: The study revealed the dose dependent increase in the scavenging activity of the extract and exhibited potent antioxidant activities. Therefore, it is suggested that the aqueous and ethanol extract of Ocimum basilicum is a source for natural antioxidant compounds and could have the potential use in the management against free radicals generated in response to oxidative stress. Keywords: Ocimum basilicum, aqueous and ethanol extract, DPPH, ABTS, hydrogen peroxide, superoxide anion, metal chelating ability
Publication date: 01/02/2022

https://ijbpas.com/pdf/2022/February/MS_IJBPAS_2022_5893.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.2.5893