ABSTRACT

Nature has been a source of medicinal agents for thousands of years and an impressive number of modern drugs have been isolated from natural sources particularly of plants, based on their use in traditional medicine. Euphorbia milii plant has been used in folk medicine in some countries like China, Nepal to treat some diseases. The present investigation was aimed to isolate and characterize the bioactive compound from methanol extract of E. milii stem which was found to be pharmacologically potent when compared with other extracts. This extract of the stem was separated into individual fractions by column chromatography and the fraction that exhibited very high antimicrobial activity against tested bacteria and fungi was elucidated to obtain the pure compound and characterized the structure. The isolated active compound was subjected to 1H NMR, 13C NMR, and Mass spectral analyses. From the spectral data obtained, the compound was identified as Quercetin-flavonoid. The isolated quercetin compound exhibited high antimicrobial activity against tested bacterial and fungal pathogens and also owned good cytotoxic activity. This research work has successfully led to the characterization of bioactive compound from the stem extract of E. milii. Keywords: Euphorbia milii, Column Chromatography, 1H NMR, 13C NMR, Quercetin, Flavonoid
Publication date: 01/02/2022

https://ijbpas.com/pdf/2022/February/MS_IJBPAS_2022_5890.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.2.5890