ABSTRACT

A simple precise, rapid and accurate reverse phase high performance liquid chromatography method has been developed and validated for the simultaneous estimation of hydrochlorothiazide and irbesartan. Shimadzu prominence i-series autosampler using Shimadzu column and separation was achieved with mobile phase acetonitrile: ammonium acetate (24:76 v/v) at flow rate 0.8 ml/min and wavelength is 290nm. Injection volume was 20 µl with 10min run time. The retention time for hydrochlorothiazide and Irbesartan is 7.488 and 2.746 min respectively. The linearity range for hydrochlorothiazide and irbesartan is 5-25 µg/ml and 6-30 µg/ml with correlation coefficient of 0.999. The %RSD for precision was found to be 0.93 and 0.92 for Hydrochlorothiazide and Irbesartan and % recovery for Hydrochlorothiazide and Irbesartan was 100.15 and 100.04 and method was robust. The developed method was validated according to ICH guidelines. Linearity, Accuracy, %RSD of precision, LOQ, LOD and robust values were found within the limits and the method was found to be satisfactory. Keywords: Hydrochlorothiazide, Irbesartan, RP-HPLC
Publication date: 01/02/2022

https://ijbpas.com/pdf/2022/February/MS_IJBPAS_2022_5889.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.2.5889