ABSTRACT

Eye ointment are commonly used to cover eyes infections. Eye ointment must be sterile when prepared. Pseudomonas aeruginosa is very common gram negative bacteria generally found to be present in ophthalmic products. It may cause serious infection of cornea. It can cause complete loss of eye sight in 24-48 hours. Eye ointments are designed to have melting point close to the human body. Eye ointment products are the most successfully sterilized dosage forms by gamma radiation due to their anhydrous nature, low melting point and because of the traditional sterilization method. Samples were purchased from different pharmacies of Lahore. The samples were enriched in nutrient broth and inoculums of these samples were cultured on nutrient agar, MacConkey agar, Potato dextrose Agar to isolate the microbial load present on them. For their identification API E20 strips were used. Upon identification the bacteria identified were bacillus subtilis. The samples were then sent to PARAS for sterilization by gamma radiation from a cobalt 60 source. Three doses of gamma radiation 15 kGy, 20kGy and 25 kGy were applied on these samples, so that the effective dose was optimized at which all the microbial flora is eliminated. The samples were again enriched in nutrient broth and then streaked on nutrient agar plates for presence of microbes separately for each dose. Samples showed no microbial growth at minimum dose which is 15 kGy. So 15 kGy is the optimized dose at which microbial load on eye ointment will be completely eliminated. Keywords: Gamma irradiation; Sterilization; microbial load; Ophthalmic ointment
Publication date: 01/02/2022

https://ijbpas.com/pdf/2022/February/MS_IJBPAS_2022_5776.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.2.5776