ABSTRACT

using assay method. The broth that showed cloudiness was noted and the optical density values are recorded using micro plate reader. After assay method the crowns were removed using automated crown remover and the abutments were removed using hex drive from all implant assembly. A plain broth was added in the inner part of implants and the samples were collected in micro centrifuge tube and values are calculated using colony forming unit method. The samples were removed and dried to confirm the bacterial presence in both restoration using SEM analysis. Mann-Whitney test was used for statistical analysis. Results: Descriptive statistics for broth turbidity values reveal that mean values for screw retained group was 0.131(SD±0.132) and cement retained group was0.3 (SD±0.016). Mann Whitney test mean ranking showed p=0.015(P<0.05).When the Colony forming unit values were compared for the microbial species individually, Mann Whitney test mean ranking in screw retained and cement retained restorations for Staphylococcus aureus were 9.80 and 11.20 respectively (p=0.591, p>0.05), for Aggregatibacter actinomycetum comitans were 9.90 and 11.10(p=0.644, p>0.05) respectively and for Porphyromonas gingivalis were 8.50 and 12.50 respectively (p=0.119, p>0.05). Conclusions: Screw retained restorations resulted in lesser bacterial penetration as compared to the cement retained restoration and there is no significant difference in penetration rate of bacteria in both the restorations. Keywords: Bacterial penetration, Implant, Screw retained, Cement retained, Microorganisms
Publication date: 25/01/2022

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https://doi.org/10.31032/IJBPAS/2022/11.1.2023