ABSTRACT

Background: Tobacco contains various known chemical component which act as direct or indirect carcinogenic agent in development oral cancer. Toxins presence in tobacco has a immunomodulatory effect. Signal transducer and activator of transcription-3(STAT3) regulates inflammation through transmitting signals from cytokines and growth factor receptor to target the genes. It has been proposed that due to tobacco carcinogen there is dysregulation and constant activation of STAT3 signaling pathway concerned in malignant transformation. In the present study, we observed expression of STAT3 in tobacco stressed mucosa and in oral Leukoplakia. To the author’s knowledge, the STAT3 expression was first time analyzed in oral mucosa stressed under various forms of tobacco and oral leukoplakia. Methods: Tissue sample of normal oral mucosa without tobacco habit (n=50) and with tobacco habit (n=50) were selected for the study to analyze STAT3 expression. Paraffin embedded tissue sections were processed for immunohistochemestry. Heamatoxylin and eosin stained slides and the presence of STAT3 in nucleus as well in cytoplasm was analyzed by two independent observers. Results: Among the 50 samples of tobacco stressed mucosa all slides shows variation in immunoreactivity. On the basis of immunorectivity score, 70% cases shows no staining i.e. score 0-2 and 30% cases shows weak expression i.e. 3-4 immunoreactive score. In comparison in different tobacco form smoking form observed to be high expression score of mean than mixed followed by smokeless which was 2.8, 2, and 0.002 respectively. No immunoexpression were observed in sample without tobacco habit. Statistical analysis were calculated by using one way ANOVA with post hoc and unpaired ‘t’ test. Conclusion: The STAT3 expression in tobacco stressed mucosa suggests its participation in modulation of molecular mechanism is the early event before obvious cellular structural changes occurs, which may further transform in to malignancy. Keywords: Signal transducer and activator of transcription-3, STAT3, tobacco habits, oral carcinogenesis, leukoplakia
Publication date: 15/01/2022

https://ijbpas.com/pdf/2022/January/MS_IJBPAS_2022_JAN_SPCL_1022.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.1.1022