FIELD SURVEY AND ISOLATION OF MICROFLORA FROM FISH Clarius batrachus Authors: S. A. Mankar And A. M. Chandak*
ABSTRACT
The beneficial and harmful microorganisms present in the gastrointestinal (GI) tract of fish.
Microflora in the gastrointestinal tract of fish is vital role for healthy living of it. It is stated
by many researchers that only few microorganisms spp. present in water are belongs to
microflora in the gastrointestinal tract. Few of these spp. acclimatizes themselves in GI tract,
whereas the remaining microorganisms are processed by digestive enzymes secreted by fish.
The molecular approach provides a more complete picture about bacterial community
composition than do cultured based methods. Application of Molecular identification based
on 16srDNA gene by using Polymerase Chain Reaction (PCR). 16S r DNA approach has
been successfully applied to determine the bacterial diversity present in GI of fish. In the
present research work, the study of molecular characterization of microflora helps in some or
another way for understanding the risk associated with pathogenic and non-pathogenic
organisms and it will also help to study the common contaminants of water isolated from the
intestine of Clarius batrachus for further research in aquaculture pathology.
Keywords: Fish, 16srDNA, PCR Publication date: 01/12/2021 https://ijbpas.com/pdf/2021/December/MS_IJBPAS_2021_DEC_SPCL_2034.pdfDownload PDFhttps://doi.org/10.31032/IJBPAS/2021/10.12.2034