ABSTRACT

Objectives: Herbal medicines are often used to provide first-line and basic health service, both to people living in remote areas where it is the only available health service, and to people living in poor areas where it offers the only affordable remedy. Punica granatum and Clerodendrum inerme Leaves have been usually used in Heart diseases, Stomach disorders, Dental care, cancer, osteoarthritis, diabetes, Anemia, liver ailments and kidney disorders. The current investigation was intended to explore the Hepatoprotective activity of a Polyherbal extract containing Punica granatum and Clerodendrum inerme leaves employing different experimental models. Methods: A comparative analysis was carried out to investigate the synrgestic hepatoprotective effect of both the plant extracts through a herbal formulation in CCl4, Paracetamol and Thioacetamide intoxicated rats. Animals were isolated into five sets, each group comprising of six rats. Methanolic extract of Punica granatum leaves (MEPGL), aqueous extract of Clerodendrum inerme leaves (AECIL) and Polyherbal extract (MEPGL+AECIL) were prepared and Hepatoprotective activity was explored against the selected models. Results: Results suggests that the serum enzymes such as SGOT, SGPT and ALP were significantly decreased with PHE (400 mg/kg p.o) when compared to toxic controls. Howeverindividual extracts of Punica granatum and Clerodendrum inerme leaves have exhibited less effect of SGOT, SGPT and ALP when compared to PHE. Total protein levels were significantly increased with PHE (400 mg/kg p.o) whereas total bilirubin and triglyceride levels were significantly decreased with PHE in comparison to individual extracts. Liver weight was significantly decreased by PHE. A histopathological examination of liver fractions has further augmented the biochemical evidences of hepatoprotective function of the Punica granatum and Clerodendrum inerme Leaf extract by preserving the hepatic architecture of the liver tissue to near normal. Conclusion: To sum up, the hepatocellular injury was prevented better with PHE of Punica granatum and Clerodendrum inerme Leaves when compared with the independent extracts indicating synergestic effect of Polyherbal extract. However further research is essential to find out the exact mechanism responsible for the synergistic hepatoprotective effect of the Polyherbal extract. Keywords: Punica granatum leaves, Clerodendrum inerme leaves, Polyherbal extract, synergistic effect, hepatoprotective activity, hepatic architecture, protein levels
Publication date: 25/09/2021

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https://doi.org/10.31032/IJBPAS/2021/10.9.1062