ABSTRACT

new, simple, sensitive, selective, rapid, and high-throughput liquid chromatographytandem mass spectrometry (LC-MS/MS) method has been developed and validated for simultaneous quantification of Telmisartan and hydrochlorothiazide in human plasma. An HPLC system (Shimadzu, Kyoto, Japan) consisting of an advance C18 column, a binary LC20AD prominence pump, an auto-sampler (SIL-HTc) and a solvent degasser (DGU-20A3) was used for the study. The separation of the analytes was carried out on an Aquasil-C18 (250×4.6mm×5µm) column. Temperature was set to 200C. The mobile phase composed of buffer solution, Methanol and Acetonitrile in the ratio of 60:20:20 (v/v) in isocratic condition at a flow rate of 0.5m L/min for 10min and the isocratic mobile phase comprised. The linearity ranges were found to be 40.064ng/ml - 801.272ng/ml for Telmisartan and 20.178ng/ml - 908.019ng/ml for Hydrochlorothiazide. Keywords: Hypertension, Telmisartan, hydrochlorothiazide, LC-MS/MS, pharmacokinetics
Publication date: 25/09/2021

https://ijbpas.com/pdf/2021/September/MS_IJBPAS_2021_SEPT_SPCL_1061.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.9.1061