ABSTRACT

The species Chlorococcum humicola had been reported to be useful for a mirage range of purposes including as arenewable source of biofuels, bioproducts and as a potential source of important bioactive compounds. However, cell multiplication and growth of the species in liquid culture media was found to be slow. It has been reported that pH is one of the key determining factor in the successful multiplication and growth of almost all micro algal species in both culture as well as natural conditions. Keeping this in mind, the present study was carried out with the objective to determine the optimum pH which favoured the maximum multiplication and growth of Chlorococcum humicola in liquid Bold Basal medium. In order to achieve this objective, the multiplication and growth performance of the species was evaluated under different conditions of pH (pH 3-10) for a period of 8 weeks or 56 days (data collected weekly). During the weekly experimental analysis, culture media characteristics like conductivity, temperature, pH and the cultured species multiplication and growth responses like cell count, cell size, turbidity and biomass were analysed. Experimentation was conducted in triplicates with a control at each specific initial pH. It has been found that the optimum pH of Bold Basal medium favouring maximum growth of Chlorococcum humicola was that of pH 8. Keywords: Chlorococcum humicola, pH, Bold Basal Medium
Publication date: 01/01/2022

https://ijbpas.com/pdf/2022/January/MS_IJBPAS_2022_5812.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.1.5812