ABSTRACT

A reverse phase liquid chromatography (RP- HPLC) method has been developed and subsequently validated as per ICH guidelines for the determination of Deferiprone in bulk and its pharmaceutical dosage form. Separation was achieved with Symmetry C8 column (4.6 x 150mm, 5m, Make: XTerra) with ambient temperature. A mixture of phosphate buffer and acetonitrile (40:60 v/v) was used as mobile phase, pH was adjusted to 3.5 with 0.1% orthophosphoric acid at a flow rate of 0.6 ml/min with a run time of 10 min by using UV detector at 285 nm. The retention time was found to be 2.976 minutes. The method was rapid, simple and sensitive. The described method of Deferiprone was linear in the range of 20- 60µg/ml with correlation coefficient of 0.999. The method enables accurate, precise and rapid analysis and it can be conveniently adopted for routine quality control analysis of bulk and pharmaceutical dosage form. Keywords: Deferiprone, Thalassaemia, Kefler, Validation
Publication date: 01/01/2022

https://ijbpas.com/pdf/2022/January/MS_IJBPAS_2022_5811.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.1.5811