ABSTRACT

Few spectraphotometric and chromatographic methods have been reported for the estimation of Vitamin C in bulk and tablet dosage form. But the there is not much more methods for assay of effervescent tablet dosage form. The following method had been developed for estimation of Vitamin C in bulk drug and effervescent formulation. Development and Validation of RPHPLC method for the estimation of Vitamin C in Bulk Drug and Dosage Form. A new RP-HPLC method was developed for assay of Vitamin C in bulk drug and formulation. The separation was achieve on C18 ODS (Hypersil), 250 nm x 4.6 mm, 5 µ using Buffer and Methanol (90:10) use as a mobile phase for estimation of Vitamin C. Detection was carried out in a UV detector at 244 nm. The validation of method was carried out using ICH guidelines. The developed method was accurate, precise, selective, and economical. The method provides sharp and proper peak hence can be employed for the estimation of Vitamin C in bulk and formulation.
Publication date: 15/12/2021

https://ijbpas.com/pdf/2021/December/MS_IJBPAS_2021_DEC_SPCL_1046.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.12.1046