ABSTRACT

Plant-based medicine gained importance due to its safety margin and multiple benefits. Regular intake of antioxidants is an alternative to avoid serious illness. The current investigation aims to measure the phenolic, flavonoid content, the antioxidant activity of Atylosia goensis Dalzell, and its antiinflammatory profile. Total phenolic content was estimated by standard colorimetric assays and antioxidant activity was screened using DPPH and Nitric Oxide free radical scavenging assay. The extracts were screened for In vitro COX enzyme inhibition assay with the two isoenzymes (COX1&COX-2) and ethanol extract was screened for In vivo anti-inflammatory activity using carrageenan induced rat paw edema model. Results disclosed that total phenolic and flavonoid content for the ethanol extract (38.24±0.72 &97.12±1.63) was found to be higher and is more effective in inhibiting the free radicalsin DPPH assay (IC50= 49.53 µg/ml) and Nitric Oxide free radical scavenging assay (IC50=41.26 µg/ml). It is also a potent inhibitor for COX enzyme and a more selective inhibitor for COX-2 (IC50= 65.14µg/ml). The ethanol extract was also reported to be safe at 2000 mg/ kg body weight and exhibited significant anti-inflammatory activity in the selected model in a dose-dependent manner at a dose of 200 and 400 mg/kg body weight. The presence of various secondary metabolites may be responsible either alone or in combination for the observed pharmacological activities. The quantity of the phenolic compounds and flavonoids can be directly correlated to the exhibited antioxidant and anti-inflammatory activities. Keywords: Atylosia goensis Dalzell, Carrageenan induced rat paw edema model, COX inhibition, Free radical scavenging, Total flavonoid content, Total phenolic content
Publication date: 25/09/2021

https://ijbpas.com/pdf/2021/September/MS_IJBPAS_2021_SEPT_SPCL_1055.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.9.1055