ABSTRACT

Fisetin (3,7,3’,4–tetrahydroxyflavone) belongs to a subgroup of flavonoids, that are widely present in the fruits and vegetables. Fisetin possess many beneficial properties which include antioxidant activities, anti-toxicity, anti-hyperlipidemic, neuroprotective and anti-proliferative actions. Colorectal cancer is the second leading cause of cancer death worldwide. In healthy cells, regulation of apoptosis plays vital role in maintaining normal colonic epithelia, and any deregulation often leads to colon cancer. The IL-6/JAK/STAT3 signaling pathway plays important role in the colorectal tumorigenesis. In IL-6/JAK/STAT signaling pathway showed increase in proliferation and survival of tumor cells while suppressing the antitumor immune response. In this study, we aimed to analyze the chemotherapeutic effect of Fisetin combined with or without 5-Fluorouracil against IL-6 stimulated HT-29 cancer cells. Cell viability was assessed using MTT assay at different concentrations. Fisetin at the concentration of 60µM (IC50) decreased the expressions of interleukin 6 (IL-6), Janus kinase 2 (JAK-2), phosphorylated Janus kinase 2 (pJAK-2), signal transducer and activator of transcription 3 (STAT3) and Nuclear factor kappa B (NF-?B) in HT-29 cells, which were confirmed by Western blot analysis. Furthermore, IL-6 stimulated HT-29 colon cells treatment with Fisetin regulates apoptotic cell death. Together, the results of this study revealed that Fisetin can act as a potent inhibitor of HT-29 cell proliferation can be used as chemopreventive drug against colon cancer. Keywords: Colon cancer, HT-29, Fisetin, IL-6/JAK/STAT3, NF-?B, apoptosis
Publication date: 01/11/2021

https://ijbpas.com/pdf/2021/November/MS_IJBPAS_2021_NOV_SPCL1130.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.11.1130