ABSTRACT

Stress is a physiological and psychological response that alters the homeostasis in the body. Cellular stress can trigger several chronic illnesses and need to be addressed in the initial stages. Polyphenolics such as tannins and flavonoids are proven powerful antioxidants to resolve cellular stress, sustain homeostasis in the body and prevent stress-associated disorders. Plant-based medicine gained importance due to its safety margin and multiple benefits. Regular intake of antioxidants is an alternative to avoid serious illness. The current investigation is aimed to measure the phenolic, flavonoid content, the antioxidant activity of Alternanthera ficoidea (L.) P. Beauv. Total phenolic content was estimated by Folin–Ciocalteu colorimetric method taking gallic acid as standard. Whereas, total flavonoid content was determined by Aluminum chloride colorimetric assay using Rutin as standard. The absorbance was measured at 760?nm and 510?nm respectively. Antioxidant activity was measured by two In vitro methods (DPPH and NO free radical scavenging assay) using standard protocols, where Ascorbic acid served as a reference standard. Results disclosed that total phenolic content and flavonoid content for the ethanolic extract of A. ficoidea are 45.61±1.08 GAE and 29±1.32 GER respectively. In DPPH assay, ethanolic extract is effective to inhibit (72.17%) the free radicals with IC50 values 45.67µg/ml at higher doses that are comparable to standard ascorbic acid (80.15%, IC50 =36.59 µg/ml). Similarly, in NO free radical scavenging assay, A. ficoidea (70.36%) exhibited free radical scavenging activity with IC50 values 45.61µg/ml. Whereas, ascorbic acid is found to inhibit 80.15% with IC50 values 36.59µg/ml. The presence of various secondary metabolites such as alkaloids, carbohydrates, phenols, steroids, terpenoids, glycosides, saponins, especially tannins and flavonoids, either alone or in combination may be responsible for the observed scavenging property. The quantity of the phenolic compounds and flavonoids can be directly correlated to the exhibited activity. Keywords: Alternanthera ficoidea (L.) P. Beauv, antioxidant, total phenolic content, total flavonoid content, free radical scavenging, amaranthaceae
Publication date: 25/09/2021

https://ijbpas.com/pdf/2021/September/MS_IJBPAS_2021_SEPT_SPCL_1034.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.9.1034