ABSTRACT

The aim of the present study was to evaluate the antibacterial and the antibiofilm activity of essential oil (EO) of Achillea Fragrantissima and its mode of action. Minimum Inhibitory Concentration (MIC) was performed in normal 96-well microtitre plates using a twofold dilution series, while, Minimum Biofilm Inhibitory Concentration (MBIC) assays were performed using a biofilm inoculator with a 96-well plate with peg lids. The results showed that Achillea Fragrantissima EO was able to overcome the resistance of all studied bacterial isolates with MIC values in the range of 0.25-1 mg/ml, while, the MBIC values were in the range of 0.50-2 mg/ml. The results showed that there were elevated leakage of potassium ions and release of cell materials in all tested bacterial strains treated with Achillea Fragrantissima EO indicating damage in the cell membrane. Additionally, it is believed that Achillea Fragrantissima EO was able to inhibit first step in biofilm formation (initial adherence) through manipulating the cell membrane of Staphylococcus epidermidis (ATCC 35984). Keywords: antibacterial, antibiofilm, Achillea Fragrantissima , mode of action, essential oil
Publication date: 01/12/2021

https://ijbpas.com/pdf/2021/December/MS_IJBPAS_2021_5753.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.12.5753