ABSTRACT

In the present study phytochemical components of vitex negundo (accession no: 3017/ Botany/St.Joseph college/Trichy) plant extract was analysed using TLC, UV, and FT-IR techniques in this study. flavonoids, saponins, cardiac glycoside, terpenoids, quinones, alkaloids, and Phlobatannins were found in preliminary phytochemical investigation. TLC was performed with chloroform, ethanol, ethyl acetate, hexane, and acetic acid in a ratio of (10:2:5:1:1) and the Rf value was determined. TLC analysis revealed the presence of a spot with an Rf value of 0.80. The presence of an absorption peak at 330 nm was confirmed by UV-Vis spectroscopy investigation. The presence of carboxylic acid, aromatic compound, and halo alkane functional groups was confirmed by FTIR analysis. The TLC, UV-VIS, and FTIR spectrum profiles of the medically significant vitex negundo plant were developed as a result of our research. Keywords: vitex negundo, TLC, UV-VIS, FTIR, Phytochemical analysis
Publication date: 01/11/2021

https://ijbpas.com/pdf/2021/November/MS_IJBPAS_2021_NOV_SPCL1073.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.11.1073