ABSTRACT

This study aimed to investigate in vitro anticancer activity of Hypsizygous ulmarius (Bull.) fruiting bodies extracts against MCF-7 breast cancer cell line. Hypsizygous ulmarius have been extracted using methanol and fractionated by petroleum ether and ethyl acetate, respectively. Phytochemical analysis using standard methods were applied. MTT assay was used to evaluated anticancer activity of methanol extract, ether and ethyl acetate fractions against MCF-7 cancer cell line. Phytochemical analysis showed the presence of alkaloids, tannins and phenolic compounds, flavonoids, anthraquinone, saponin, coumarin steroids in methanol extract. Petroleum ether fraction contain tannins and phenolic compounds which are absent in ethyl acetate fraction. Anticancer activity using MTT assay showed that ethyl acetate fraction the maximum activity against MCF-7 cancer cell line with IC50 value of 10.78 ?g/ml followed by extract with IC50 value of 24.92 ?g/ml. The lowest activity was showed by petroleum ether fraction with IC50 of 39.98 ?g/ml. Keywords: H. ulmarius, MCF-7, MTT assay, Methanol extract, Petroleum ether fraction, Ethyl acetate fraction
Publication date: 01/11/2021

https://ijbpas.com/pdf/2021/November/MS_IJBPAS_2021_5717.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.11.5717